Scheduling canine breeding is particularly difficult. Kisspeptin infusion in other species has stimulated LH secretion and triggered ovulation. The purpose of this study was to gain evidence as to the feasibility of using kisspeptin treatments as a means of stimulating follicular development and hence ovulation in anoestrous bitches. Five pairs of clinically normal, mature anoestrous Greyhounds were used in the study, with each bitch in a pair randomly allocated to receive either treatment (kisspeptin injections; 0.5nMol/kg/injection) or control (normal saline; 0.5mL). Injections were given intravenously every 4h for 10 days. To investigate the effect of treatment on pulsatile LH secretion, blood samples were collected every 10 minutes for the first 8 hours with the first injection given after 3h. Samples were then collected every 12h until the completion of the study to determine if an LH surge was generated. An LH assay was performed using a commercial ELISA kit. Preliminary results indicate little increase in LH pulsatility and no LH surge following kisspeptin treatment, a result supported by post-trial serum progesterone measurements. Despite this, the ovaries from treatment dogs showed follicular development with numerous antral follicles, whilst the ovaries of the controls were smooth without any structures. Two of the treatment dogs entered pro-oestrus during the treatment period, with clear signs of vulval oedema and serosanguinous discharge, supported by vaginal cytology. This may imply that the kisspeptin treatment protocol used in this study can stimulate FSH and initiate follicular development in an anoestrous bitch, but cannot induce an LH surge and ovulation. Either a higher dose of kisspeptin treatment or addition of an ovulation induction agent to the current protocol may lead to LH surge and ovulation.