Insulin is a vital molecular component of mammalian glucose control with resting levels changing significantly in patients suffering from diabetes mellitus. As a sole genetic cause of diabetes has been ruled out and some research is leading towards epigenetic causes; understanding the impact to the pre-implantation embryo is becoming a major component of diabetic research. Many of these studies are performed in vitro and interestingly, to date, no known studies have investigated the effect of varying levels of insulin and have only focused on varying glucose levels. The research performed here studies embryonic development using cumulus oocyte complexes derived from PMSG stimulated mice at 46 h before undergoing in vitro maturation (IVM) in varying levels of insulin (0.17pM, 1.7pM, 1700pM and 17000pM). IVM is also performed at these insulin levels both with and without the presence of glucose (30mM) to mimic different stages and types of diabetes mellitus, before in vitro-fertilisation occurs. Analysing cumulus expansion using the cumulus expansion index (17h post FSH in vitro), cleavage rate at 2-cell (24 hours post fertilisation) and blastocyst rate (4 days post fertilisation) we identified that the presence of insulin significantly reduced the cleavage rate at all concentrations compared to the control without insulin (p=0.49), while only significantly reducing cumulus expansion at the lowest level of 0.17pM (p=0.038). There was no effect of either insulin or glucose on blastocyst rate; however the addition of high glucose resulted in a significantly higher cumulus (p=0.047) expansion score and a lower cleavage rate (p=0.002) supporting previous studies work. These findings suggest that considering insulin as well as glucose in epigenetic research of diabetes may be a priority and provides reasoning to further investigate the causes of the impact of insulin such as; gene expression and metabolic activity.