Poster Presentation ESA-SRB Conference 2015

INSR rs2059806 polymorphism and the risk of preeclampsia (#308)

Prabha Andraweera 1 , Kathryn Gatford 1 , Gustaaf Dekker 1 2 , Shalem Leemaqz 1 , Steven Thompson 1 , Lesley McCowan 3 , Vajira Dissanayake 4 , Rohan Jayasekara 4 , Claire Roberts 5
  1. Discipline of Obstetrics and Gynaecology, Robinson Reearch Institute, University of Adelaide, Adelaide, SA, Australia
  2. Women's and Children's Division, Lyell McEwin Hospital, Elizabeth Vale, Australia
  3. Department of Obsterics and Gynaecology, University of Auckland, Auckland, New Zealand
  4. Human Genetics Unit, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka
  5. Discipline of Obstetrics and Gynaecology, Robinson Research Institute, University of Adelaide, Adelaide, SA, Australia

Introduction: Preeclampsia is a pregnancy specific disease that occurs in 2-8% of pregnancies and is a leading cause of maternal morbidity and mortality. Increasing evidence suggests that the effects of preeclampsia on a woman’s health are not restricted to the pregnancy but that preeclampsia could represent a risk factor for later life vascular and metabolic diseases. The INSR rs2059806 single nucleotide polymorphism (SNP) is a risk factor for essential hypertension, type 2 diabetes and metabolic syndrome. We investigated the association of this polymorphism with preeclampsia.

Methods: The association of the INSR rs2059806 SNP with preeclampsia was tested in 123 Caucasian preeclamptic women and 1185 controls and replicated in an independent cohort of 175 Sinhalese preeclamptic women and 171 controls. The Caucasian women were recruited from the SCOPE study in Adelaide and Auckland and the Sinhalese women were recruited in Colombo, Sri Lanka. Preeclampsia was diagnosed using international guidelines. The controls consisted of women who had uncomplicated pregnancies. DNA was extracted from peripheral blood collected from women and was genotyped using the Sequenom MassARRAY system. The genotype frequencies of the preeclamptic women were compared with controls using chi squared test.

Results: In the Caucasian cohort, the prevalence of the INSR rs2059806 AA genotype was increased among preeclamptic women [OR(95%CI)=3.1(1.6-5.8), p=0.0003]. In the Sinhalese cohort, the prevalence of the INSR rs2059806 AA genotype was increased among preeclamptic women who delivered small for gestational infants [OR(95%CI)=2.8(1.0-7.4), p=0.03].

Conclusion: The INSR rs2059806 SNP previously associated with adult vascular and metabolic diseases is also associated with preeclampsia in two independent cohorts. These findings suggest that genetic susceptibility may be implicated in the link between preeclampsia and subsequent vascular and metabolic diseases.